Open system metabolism, nutrient uptake as well as bacterial abundance, sediment organic matter content and hyporheic respiration were examined for sub-alpine spring brooks during summer 2004 in the Swiss Alps. Whole system metabolism was estimated by the dualstation diel oxygen method. Liv23b, PA1 and PP11 had average gross primary production (GPP) of 4.2, 2.4 and 4.3 g O2 m-2 d-1, respectively. Measurement for ecosystem respiration (ER) averaged 5.8, 3.6 and 6.7 g O2 m-2 d-1 for Liv23b, PA1 and PP11, respectively. GoFuO had an average GPP of 65 g O2 m-2 d-1 and an ER of 96 g O2 m-2 d-1. Mean phosphorus and nitrogen uptake length ranged from 5 to 46 m and 20 to 63 m, respectively. Assessment of uptake rates averaged from 0.3 to 3.1 μg m-2 h-1 and 57 to 178 μg m-2 h-1 for P and N, respectively. Sediment samples were stained with 4’, 6-diamidino-2-phenylindole (DAPI)-solution and subsequent counting of bacteria. Counts of DAPI-stained cells per mL sediment ranged from 1.1 x 106 to 3.4 x 106.