Propelled by the rapid development of equipment, technology and computational power, the monitoring and simulation of the hydrodynamics in lakes have steadily advanced. In contrast, water quality simulations are more difficult to implement, due to the difficulty in obtaining large-scale, spatially resolved field observations for model validation and the number of interacting processes to be parameterized. Here we demonstrate that remote sensing data can be used to inform Lagrangian particle tracking in a large lake, and vice versa. We used total suspended matter (TSM) as a parameter that can be both estimated from the backscattering in satellite images and modelled in terms of particle abundance. Specifically, we compared TSM concentrations in Lake Geneva deduced from images taken by Sentinel-2 and Sentinel-3 satellites to those estimated from Delft3D hydrodynamic and particle tracking models. TSM concentrations obtained from both methods were compared over a time span of up to 5 days in several scenario studies, including instantaneous and continuous point sources and large-scale TSM simulations. The results demonstrate that remote sensing images can serve to calibrate and validate particle tracking models with independent observations. The model was able to capture both the position of a TSM cloud arising 5 days after an instantaneous point source release, and the direction of particle transport and TSM plume size resulting from a continuous source. Even when simulating the whole lake domain, model results closely approximated the satellite-derived TSM concentrations along lake transects within 9%. In return, the particle tracking model was able to complete partially impaired satellite images, and fill in a four-day image gaps between satellite revisits. The synergy of remote sensing techniques and particle tracking modelling allows a rapid, continuous and more accurate analysis on solute transport in lakes.