Benzo(a)pyrene (BaP) is a ubiquitous pollutant derived by incomplete combustion of organic matter. BaP is classified by the International Agency of Cancer (IARC) as carcinogenic to humans and is controlled in drinking water in the United States and Europe. BaP needs to be metabolized to become carcinogenic. Its metabolism is induced by the interaction between  BaP and the aryl hydrocarbon receptor (AhR) and subsequent gene regulation. Despite this general knowledge, details about BaP entry into cells, distribution and internal concentrations able to elicit a cellular response are largely unexplored and mechanisms of actions incompletely understood. This PhD thesis therefore aims to investigate in a time and concentration dependent manner the molecular response of a murine hepatoma cell line, Hepa1c1c7, exposed to BaP, combining high throughput techniques, bioinformatics, phenotype characterization and chemical analysis. [...]