Acoustic Emission (AE) has the potential to identify delamination initiation in quasi-static Mode I fracture tests on glass fiber-reinforced polymer-matrix (GFRP) laminates. It had been shown that a combined AE activity and intensity criterion yields critical Mode I delamination resistance values GIC for an AS4/PEEK carbon fiber thermoplastic composite that are comparable to those from data analysis according to ASTM D5528. However, the AE historic index also allows for detection of delamination initiation under Mode I and Mode II loading of carbon fiber reinforced thermoplastics and thermosets. In the present study, these AE criteria for initiation under Mode I, Mode II and Mixed Mode I/II loading of GFRP epoxy laminates are compared with AE signal energy as additional initiation criterion. Initiation under Mode II and Mixed Mode I/II at a ratio of 4:3 was determined with two different setups for each Mode. For some test configurations noise signals make unambiguous initiation detection difficult. Determination by means of AE energy or AE activity and intensity yields more conservative GC values compared with the AE historic index or criteria defined in the standards, however, with similar repeatability.