Laser-Powder Bed Fusion (L-PBF) techniques have revolutionized the production of Ti6Al4V alloys across various industries. However, the widespread adoption of L-PBF Ti6Al4V alloys is impeded by their inadequate fatigue performance, particularly in high cycle regimes. A significant contributing factor to this limitation is the presence of internal defects inherent to the L-PBF process, act as sites for fatigue crack initiation. Previous investigations have focused on the fatigue performance of L-PBF Ti6Al4V alloys with gas porosities, while research on lack-of-fusions (LOFs) which are recognized as the most detrimental defects, remains limited. In order to deepen our understanding of the factors influencing the reduced fatigue performance observed in L-PBF Ti6Al4V alloys associated with inherent LOFs, this study employed three-dimensional (3D) finite element analysis approaches. Such approaches allow to assess fatigue indicator parameters to evaluate fatigue life and predict fatigue crack propagation. A 3D average Smith-Watson-Topper method has been proposed, which is able to rationally estimate the fatigue life of L-PBF Ti6Al4V. In addition, a novel finite element method has been developed to accurately calculate stress intensity factors along irregular shaped crack front of a notch-like feature embedded on a LOF. Additionally, parametric studies were conducted to gain further insights into the influence of LOFs on fatigue performance. The results shown the presence of embedded humps and notch-like features, and their topologies play key roles in the fatigue performance of LOF predominated L-PBF Ti6Al4V alloys.