The number of time-of-flight secondary ion mass spectrometry studies on biological tissues and cells has strongly increased since the development of primary ion sources that allow not only elemental but also molecular analysis. Substantial fragmentation during ionic bombardment results in a large number of peaks, rendering data analysis complex. Complete and trustable sets of reference spectra for the main biological building blocks, i.e., amino acids, monosaccharides, fatty acids, and nucleotides, are required. This work aims to provide an accurate and extensive library of reference spectra for monosaccharides, measured with the Bi₃⁺ primary ion. Here (Paper II), the positive polarity spectra and lists of associated characteristic fragments are presented.