This research describes the sustainable separation and identification of Gallic acid (GA) from herb using circularly spreded silica gel layer by chromatographic separation method. The purity of isolated Gallic acid confirmed by reverse phase High-performance liquid chromatography (HPLC) which was 98.24%. Compound was isolated and identified as gallic acid through Ultraviolet–visible spectroscopy (UV-Vis.), Fourier-transform infrared spectroscopy (FTIR), HPLC, High-resolution mass spectrometry (HR-MS); 1H, 13C Nuclear magnetic resonance (NMR) and Differential scanning calorimetry (DSC) measurements. This method was reported first time for Gallic acid isolation from F. arnottiana leaves. Gallic acid is mainly used in synthesis of polymers and grafting process. Gallic acid exhibited higher levels of antioxidant activity hence it is an ideal choice for reducing the risk of cancer.