Early detection of volcanic eruptions is of major importance for protecting human life. Ground deformation and changes in seismicity, geochemistry, petrology, and gravimetry are used to assess volcanic activity before eruptions. Studies on Mt. Etna (Italy) have demonstrated that vegetation can be affected by pre-eruptive activity before the onset of eruptions. During two consecutive years before Mt. Etna's 2002/2003 flank eruption, enhanced vegetation index (NDVI) values were detected along a distinct line which later developed into an eruptive fissure. However, the mechanisms by which volcanic activity can lead to changes in pre-eruption tree growth processes are still not well understood. We analysed δ¹³C, δ¹⁸O and ¹⁴C in the rings of the survived trees growing near to the line where the pre-eruptive increase in NDVI was observed in order to evaluate whether the uptake of water vapour or fossil volcanic CO₂ could have contributed to the enhanced NDVI. We found a dramatic decrease in δ¹⁸O in tree rings formed before 2002/2003 in trees close to the eruption fissure, suggesting uptake of volcanic water by trees during pre-eruptive magma degassing. Moist conditions caused by outgassing of ascending magma may also have led to an observed reduction in tree-ring δ¹³C following the eruption. Furthermore, only ambiguous evidence for tree uptake of degassed CO₂ was found. Our results suggest that additional soil water condensed from degassed water vapour may have promoted photosynthesis, explaining local increases in NDVI before the 2002/2003 Mt. Etna flank eruption. Tree-ring oxygen stable isotopes might be used as indicators of past volcanic eruptions.