Mass-spec, cryo-EM and AI join forces for a close look at the transporter complex in cilia

Ishikawa T
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Ishikawa, T. (2023). Mass-spec, cryo-EM and AI join forces for a close look at the transporter complex in cilia. EMBO Journal, 42, e113010 (3 pp.). https://doi.org/10.15252/embj.2022113010
https://www.dora.lib4ri.ch/psi/islandora/object/psi:49146

The intraflagellar transport (IFT) complex transports components between the cytoplasm and the ciliary tip. Two studies now report on the atomic structure of IFT-B, the core of IFT, using cutting-edge technology, such as cross-linking mass spectrometry (MS), cryo-electron tomography (cryo-ET) and Alphafold2-enabled AI-based folding prediction. The 3D structure of IFT-B reveals how the 15 component proteins are arranged to stabilize this gigantic complex and suggests a dynamic interplay between the proteins.

Details
Publication Type

Journal Article

Title

Mass-spec, cryo-EM and AI join forces for a close look at the transporter complex in cilia

Author(s)

Ishikawa, Takashi (2412 Cellular Structure Imaging)

Journal

EMBO Journal

Volume

42

Start Page

e113010 (3 pp.)

Publication Year

2023

Peer Review

Yes

Funding (EC, SNSF)

Swiss National Science Foundation: Structural study on calcium regulation of motile cilia by cryo-EM (192644)

Collection

PSI Publications
Open Access