Water management is a key factor in the operation of hydrogen fuel cells since its formation may lead to significant mass transport losses, oxygen diffusion limitation and membrane durability issues. In this work, the effect of different operating conditions on the liquid water distribution inside a 50 cm² active area bio-inspired PEM fuel cell has been studied. Therefore, a set of experiments was designed varying cell pressure, the reactants relative humidity (anode and cathode), temperature, and cell current density. Liquid water distribution for each operating condition was determined using neutron imaging technique as it has been proved to be an excellent technique for this purpose, including quantitative analysis and water profiles in the different areas of the bio-inspired flow field. The results show that high relative humidity of the inlet gas flows, high pressure, low temperatures and low current density favor the accumulation of water in the flow field channels and GDL. Specifically, water accumulates preferentially in the anode side that make contact with the low part of the cathode foams inserted in the flow field, instead of blocking the closest area to the gases outlets points. This is due to permeation from the cathode foams to the anode side.