High precision experiments with muons and pions often require tracking charged particles with O(100 μm) single-hit resolution, possibly with particle identification capabilities, down to very low momenta (p ≲ 100 MeV/c). In such conditions, the particle trajectories are strongly affected by the interaction with the detector material, and the reconstruction of the kinematic observables consequently deteriorates. A good compromise between resolution and material budget can be obtained with a Time Projection Chamber (TPC), if very light gases and a high-granularity readout are used. In this paper, we present a characterization of the GridPix detector in helium-isobutane gas mixtures, within a TPC with 9 cm maximum drift. Measurements of the main electron drift properties for these gas mixtures are also presented.