Localized measurement of the impedance distribution in an operating polymer electrolyte fuel cell stack is of great importance in providing information on its state of health and performance. However, the current methods’ invasiveness is a brake to a more widespread utilization. This work presents a non-invasive way to measure and interpret local impedance spectra. It is based on local AC current supply and local voltage measurements performed all around the cell via conductive pins contacting the outer surface of the flow fields. The different local spectra are measured on a single cell within a 200 cm² six-cell stack under different operation conditions and compared to a reference case with homogeneous current density. A simple equivalent electrical circuit is used to fit the impedance spectra and to extract membrane and charge transfer resistance. The variations of these two values are analyzed at five different positions along the channel for different cathode stoichiometries and gas flow configurations.