This chapter discusses recent progress in 2D and 3D printing technologies, in particular laser-induced forward transfer and two-photon polymerization (TPP). We explore their potential for applications in micro-electronics, protein microarrays, sensors and biosensors, and tissue engineering. An overview of the factors that affect patterning, miniaturization and functionality is presented. A special focus is placed on laser direct writing via TPP for the fabrication of 3D vertical microtubes acting as microreservoirs for an osteogenic drug.