Zinc (Zn) deficiency is a major problem for many people living on wheat-based diets. Here, we explored whether addition of green manure of red clover and sunflower to a calcareous soil or inoculating a non-indigenous arbuscular mycorrhizal fungal (AMF) strain may increase grain Zn concentration in bread wheat. For this purpose we performed a multifactorial pot experiment, in which the effects of two green manures (red clover, sunflower), ZnSO ₄ application, soil γ-irradiation (elimination of naturally occurring AMF), and AMF inoculation were tested. Both green manures were labeled with ⁶⁵Zn radiotracer to record the Zn recoveries in the aboveground plant biomass. Application of ZnSO₄ fertilizer increased grain Zn concentration from 20 to 39 mg Zn kg^-1 and sole addition of green manure of sunflower to soil raised grain Zn concentration to 31 mg Zn kg^-1. Adding the two together to soil increased grain Zn concentration even further to 54 mg Zn kg^-1. Mixing green manure of sunflower to soil mobilized additional 48 μg Zn (kg soil)^-1 for transfer to the aboveground plant biomass, compared to the total of 132 μg Zn (kg soil)^-1 taken up from plain soil when neither green manure nor ZnSO ₄ were applied. Green manure amendments to soil also raised the DTPA-extractable Zn in soil. Inoculating a non-indigenous AMF did not increase plant Zn uptake. The study thus showed that organic matter amendments to soil can contribute to a better utilization of naturally stocked soil micronutrients, and thereby reduce any need for major external inputs.