Variations on a theme: crystal forms of the amino-acid transporter MhsT

Neumann, Caroline; Focht, Dorota; Trampari, Sofia; Lyons, Joseph A.; Nissen, Poul

doi:10.1107/S2053230X22007154

Variations on a theme: crystal forms of the amino-acid transporter MhsT

Neumann C, Focht D, Trampari S, Lyons JA & Nissen P

Citation

Neumann, C., Focht, D., Trampari, S., Lyons, J. A., & Nissen, P. (2022). Variations on a theme: crystal forms of the amino-acid transporter MhsT. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 78, 297-305. https://doi.org/10.1107/S2053230X22007154

Persistent URL https://www.dora.lib4ri.ch/psi/islandora/object/psi:50231

The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT-substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational noncrystallographic symmetry.

Details

Publication Type

Journal Article

Title

Variations on a theme: crystal forms of the amino-acid transporter MhsT

Author(s)

Neumann, Caroline

Focht, Dorota

Trampari, Sofia (2450 Mechano-Genomics)

Lyons, Joseph A.

Nissen, Poul

Journal