Natural abundance (δ) of the stable isotopes ¹³C and ¹⁵N has gained acceptance for studying C and N cycling in forests. In most studies, bulk soil samples are collected to determine isotope abundance. Such sampling overlooks the potential impact of preferential flow on isotope distribution. The objective of this study was to investigate the effects of preferential flow on the distribution of soil organic carbon (SOC), total N, δ¹³C, and δ¹⁵N in a forest soil in Central Switzerland. Preferential flow paths in the soil were identified with a dye tracer, Brilliant Blue (Plüss‐Staufer AG, Oftringen, Switzerland), that was homogeneously applied to the soil surface. In the stained preferential flow paths, concentrations of SOC and total N were 15 to 75% higher than in the soil matrix. The total increase of SOC in preferential flow paths ranged from 740 to 960 g C m⁻² in four individual soil plots. Values of δ¹³C and δ¹⁵N were lowest in tree leaves and in the forest floor, and increased with soil depth, thus with the degree of decomposition of SOC. In the mineral soil, preferential flow paths were significantly depleted in ¹³C by 0.15 to 0.4‰ as compared with the soil matrix. The δ¹⁵N values increased with soil depth from 0.9 to 4.7‰ in the preferential flow paths and from 0.5 to 6‰ in the soil matrix. Adding a highly enriched ¹⁵N‐tracer homogeneously to the soil surface showed a higher recovery of ¹⁵N in the soil and in the fine roots sampled from preferential flow paths than in those sampled from the soil matrix. Our results suggest that in preferential flow paths, SOC is younger and N cycling is more rapid than in the soil matrix.