Tree-related microhabitats are important for forest biodiversity. Water-filled tree holes are one such microhabitat and can be abundant in temperate forests. The arthropod community in this microhabitat not only contribute to forest biodiversity but also provides food for terrestrial predators such as arthropods, small mammals and birds. The extent of the threat of attack from terrestrial predators on insect larvae in this microhabitat, however, is poorly known. To measure predation in this microhabitat, we produced fake prey resembling insect larvae using white plasticine and exposed them at the aquatic-terrestrial habitat interface. We recorded: (1) which predators attacked the fake larvae, (2) the predation probability on the fake larvae after two days and after two weeks and (3) whether predation probability on fake larvae differed between managed and unmanaged forest zones in one of the last primeval beech forests, the Uholka division of the Carpathian Biosphere Reserve in the Ukrainian Carpathians. By addressing these questions, we aimed to quantify the predation pressure of terrestrial predators on insect larvae in tree-holes. The probability that a fake larva in a tree hole was attacked by predators ranged between 25-58% (95% CI) after two days and between 76-96% (95% CI) after two weeks. Overall, the highest attack rates were recorded for small mammals, followed by arthropods and birds. Arthropods took longer to detect potential prey items than small mammals and birds, and they were the only group that showed significant differences in attack rates between forest zones (unmanaged > managed). This study revealed that sentinel prey might be a suitable method for measuring the predation pressure from terrestrial predators on insect larvae in water-filled tree holes.