To preserve multi-centennial length variability in annual tree-ring chronologies, the Regional Curve Standardization (RCS) method calculates anomalies from a regionally common, non-climatic age-trend function. The influence of various factors on the estimation of the regional curve (RC) and resulting RCS-chronology is discussed. These factors are: the method of calculating anomalies from the age-trend function, estimation of the true pith offset, the number of series used, species composition, and site characteristics. By applying RCS to a collection of millennium-length tree-ring data sets, the potential and limitations of the RCS method are investigated. RCS is found to be reasonably robust with respect to tested factors, suggesting the method is a suitable tool for preserving low-frequency variance in long tree-ring chronologies.