Segregation of isoenzymes was studied among 34 full-sib families of Fagus sylvatica L. by means of gel electrophoresis. Of the 16 enzyme systems analyzed, two showed substantial tissue-specific expression of isoenzymes. The remaining 14 enzyme systems are controlled genetically by at least 20 polymorphic gene loci, three of which were inferred from additional population studies. The inheritance of the complex system of 6PGDH is studied in detail. A 20-locus nomenclature is suggested, including 78 codominant alleles. Analyses of two-locus combinations did not reveal linkage between any of the tested gene loci.