The interaction of small-scale vortical structures with the surrounding fluid are studied using a fully resolved three-dimensional experimental data set of homogeneous turbulence measured at the centre of a von Kármán mixing flow facility and a direct numerical simulation (DNS) data set of forced isotropic turbulence. To identify the small-scale vortices and their boundaries, an objective observer-independent definition was implemented to avoid arbitrariness and is the first implementation applied to experimental measurements of small-scale turbulence. Volume-averaged and conditional statistics are presented to demonstrate consistency between the experimental and DNS data sets. To examine the interaction of the structures with the surrounding flow field, we examine the flow across the boundary of vortex structures by adopting a similar methodological approach to that used to investigate the local entrainment and detrainment across the turbulent–non-turbulent interface. The probability density function (p.d.f.) of entrainment velocity conditioned on the vortex boundary exhibited a non-Gaussian distribution that skewed slightly in favour of entrainment and is remarkably similar to the p.d.f.s of entrainment velocity observed in boundary layers and jets. We analyse the enstrophy transport equation conditioned on radial and axial coordinates of the vortices to quantify the inviscid and viscous components of the entrainment/detrainment process. A comparison with Burgers vortices is made and it is found that the Burgers vortex model captures the vortex structure average size and the mechanisms of enstrophy transport in the radial direction, but is unable to capture local statistics and describe the governing physics along the axes of the vortices.