Using the 454 pyrosequencing-based technique in the development of nuclear microsatellite loci in the alpine plant &lt;em&gt;Arabis alpina&lt;/em&gt; (Brassicaceae)

Buehler, Dominique; Graf, René; Holderegger, Rolf; Gugerli, Felix

doi:10.3732/ajb.1000488

Using the 454 pyrosequencing-based technique in the development of nuclear microsatellite loci in the alpine plant Arabis alpina (Brassicaceae)

Buehler D, Graf R, Holderegger R & Gugerli F

Citation

Buehler, D., Graf, R., Holderegger, R., & Gugerli, F. (2011). Using the 454 pyrosequencing-based technique in the development of nuclear microsatellite loci in the alpine plant Arabis alpina (Brassicaceae). American Journal of Botany, 98(5), e103-e105. https://doi.org/10.3732/ajb.1000488

Persistent URL https://www.dora.lib4ri.ch/wsl/islandora/object/wsl:3884

Premise of the study: Polymorphic microsatellite markers were developed for the inbred alpine perennial plant Arabis alpina to infer life-history parameters and measure patterns of contemporary gene flow within populations.
Methods and Results: Using the 454 pyrosequencing technique, 19 microsatellite primer sets were developed for A. alpina. The primer sets were tested on 60 individuals sampled from three sub-populations in the Swiss Alps. The primers amplified di- and trinucleotide repeats with two to five alleles per locus.
Conclusions: Previous attempts to isolate microsatellite loci in A. alpina using enrichment libraries and cross-amplification were difficult and produced an insufficient number of polymorphic microsatellite loci. In contrast, next-generation sequencing technology was successful in identifying microsatellite repeats in A. alpina. These newly developed microsatellite primers will be useful to further develop A. alpina into a model species for eco-genomic studies.