This article describes an investigation on runoff generation at different scales in the forested catchment of the Sperbelgraben in the Emmental region (Swiss Prealps) where studies in the field of forest hydrology have a history of 100 years. It focuses on the analysis of soil profiles and the subsequent sprinkling experiments above them (1 m²), as well as on surface runoff measurements on larger plots (50 to 110 m²). In the Sperbelgraben investigation area, two very distinct runoff reactions could be observed. On the one hand, very high production of saturation overland flow was registered on wet areas of gleyic soils, with runoff coefficients between 0.39 and 0.94 for profile irrigation. On the other hand, almost no surface runoff was measured on Cambisols, with the exception at some sites of a hydrophobic reaction detected at the beginning of storms after dry periods (coefficients for profile irrigation: 0.01-0.16). This pattern was observed during 1 m², Soil plot irrigation and on surface runoff plots. Apart from a less distinctive signal of the water-repellent litter layer on the larger surface runoff plots, the dominant hydrological processes at the two scales are the same. The determined runoff reaction at the two scales corresponds well with information from a forest site type map describing soil and vegetation characteristics and used as a substitute for a soil map in this study. Theoretical considerations describing forest influence on flood discharge are discussed and evaluated to be in good agreement with observations. These findings are a sound foundation for application in hydrological catchment modelling.