Academics and city administrations generally agree that environmental management decisions should be science based, which suggests the value of collaboration between city officials and researchers. Such collaboration, termed "ecology with cities", is an example of translational ecology that should integrate ecological and social sciences to inform decision-makers. However, there has been insufficient reflection on whether ecology with cities achieves the expected development of practical social-ecological knowledge for the common good. We addressed this gap by asking city officials and researchers, in Switzerland and with whom we have collaborated in the past, about their motivations for, and experiences with, transdisciplinary collaboration. The respondents reported largely overlapping goals and an awareness of the mutual benefits of accessing the skills and resources of the other group. However, the reflections also unearthed latent tensions related to insufficient mutual awareness of institutional boundaries and limitations. We conclude that researchers should try to include collaboration partners who have experience in translational ecology practice and should establish learning processes early in a collaboration. Building good working relationships with city administrations and establishing such processes would facilitate the creation of realistic mutual expectations in which institutional limitations are considered so that common goals of maintaining or improving the ecological quality of cities can be amicably reached.